ORPHOLOGICAL characters of plantlets in vitro and ex vitro Plantlets from in vitro have shortest shoots and roots and lowest numbers of leaves and roots

Date palm tissue culture technique aimed to production of huge numbers of plants to farmers in order to increasing dates which have high nutritional value. Date palm plantlets from pre-acclimatization stage in vitro were transferred to acclimatize in the greenhouse cultured in peat moss + perlite 2:1 and kept in the plastic covered inside 100% humidity and 28 C0 till new leave or root was grown. The plantlets of date palm in vitro technique facing problem in the acclimatization stage as high mortality concerning failure in this stage, in vitro condition saturated atmosphere, relatively low light intensity, constant temperature 20-28 C0, high humidity, water continuously found, this in vitro condition lead to deficiency of plants morphology, physiology and anatomy as tiny shoots and leaves, small leaves and roots, low rates of photosynthesis, gas exchange, lowest contents of pigments, absent of cuticular wax and epicuticular, Limited palisade layer, impaired stomata, roots epidermal and subepidermal layer could not regular, cortical cells smaller in size and impaired root function. Date palm plantlets when transferred to ex vitro become stressful by different condition compared in vitro, these plantlets must be acclimatize in the greenhouse for modified these morphology, physiology and anatomy effects in order to increasing survive percent of plantlets for allowing enormous numbers of plants to farmers. Keywords— acclimatization, anatomy, date palm, ex vitro, morphology and physiology.